Testing methods

We conduct all tests based on modern methods of molecular biology such as: PCR, RFLP, Real-time PCR and sequential analysis DNA. These methods are standards in modern diagnostics and they indicate directions of its development. They provide necessary and objective results allowing us to identify clearly individual pathogens in an easy and quick way. Thanks to them, we can also establish a profile of specific regions of human DNA responsible for genetic diseases, susceptibility to cancers or defining individual relationship.

PCR i.e. polymerase chain reaction is the most sensitive known analytical method. Benefits resulting from conducting of the diagnostic test using PCR method include, among other things:

  • sensitivity – incomparable with other diagnostic methods – enables diagnosis of the infection at a very early stage regardless of antibody presence;
  • certainty – we always obtain an undoubted result – without unclear or intermediate results on the border of test sensitivity. In addition, the possibility of creating falsely negative (in approx. 10% of the virus carriers no antibodies develop in spite of the infection) and falsely positive results (appearance of antibodies is not always an evidence of the existing infection – they may appear e.g. after receiving of a vaccination or they may remain in the organism for many years after elimination of  microorganism. Apart from this, this test is independent of the presence of other pathogens – no cross reactions take place.) are limited to an absolute minimum;
  • possibility of direct identification of microorganisms – presence of genetic material (DNA or RNA) of the microorganism (bacteria, virus, protozoan etc.) is tested. It is also important that the clinical material can be taken directly from the place changed by the disease which gives a chance of recognising a local pathogenic factor (ulceration, so called erosion etc.);
  • possibility of simultaneous identification of several pathogens during one test;
  • non-invasive or low-invasive sampling of the material for testing and the possibility of controlling quality of any sampled material;

The real-time PCR is a variant of the conventional PCR method and enables analysis of the product volume growth in real time. Additional diagnostic benefits of the test conducted using this method are mainly as follows:

  • possibility of determining the number of the microorganism copies in the sampled material which is one of the most important tools for monitoring effectiveness of the applied therapy and condition of the patient before and after treatment. It allows us to control progress of treatment and reduction of its costs;

shortening the time of analysis and waiting for the result which is particularly important during testing of pathogenic microorganisms where the time of making the diagnosis is often a key factor.

laboratorium genetyczne diagnostyczne DNA
Ogólnopolskie Centrum Genetyczne Rex Company S.A.
ul. Robotnicza 32, 53-608 Wrocław
tel. 71 373 56 57, fax 71 359 06 58
e-mail: biuroobslugi@rex.pl
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