The PCR reaction with the analysis of product volume growth in real time (Real-Time PCR). Marked probes (e.g. using fluorescent dyes) combined with DNA are introduced to the reaction and based on readings in subsequent cycles of the reaction they allow us to determine the number of matrix (output DNA) used for the reaction, and also to track growth of the generated product.
This method is used, among other things, to measure the number of virus or bacteria particles in clinical materials. RealTime PCR is the best tool to monitor progress of treatment for patients. Determination of the output level of viremia or bacteraemia is an indication necessary to begin an appropriate treatment.